Journal: Journal of Medical Virology

Article Title: Construction and characterization of two SARS‐CoV‐2 minigenome replicon systems

doi: 10.1002/jmv.27650

Figure Lengend Snippet: Inhibition of IVT‐CoV2‐Rep by antiviral compounds. CHO ‐ K1 cells were transfected with 200 ng IVT‐CoV2‐Rep RNA in a 96‐well plate and subjected to the following treatments: (A) Solvent control (0.1% DMSO or H 2 O); (B) 5 μM remdesivir or GC376 with DMSO as a control; and (C) 5 μM EIDD‐1931 with H 2 O as a control. NLuc activities were measured at indicated time points (mean ± SD, n = 2). DMSO, dimethyl sulfoxide; IVT, in vitro transcription; RLU, relative luminescence unit

Article Snippet: SARS‐CoV‐2 antiviral compounds remdesivir, GC376, and EIDD‐1931 were purchased from SelleckChem.

Techniques: Inhibition, Transfection, Solvent, Control, In Vitro

Journal: Journal of Medical Virology

Article Title: Construction and characterization of two SARS‐CoV‐2 minigenome replicon systems

doi: 10.1002/jmv.27650

Figure Lengend Snippet: Antiviral treatment of BAC‐CoV2‐Rep replication. CHO‐K1 cells were transfected with BAC‐CoV2‐Rep, followed by treatment with 10 μM remdesivir, GC376, or EIDD‐1931. 0.1% DMSO or H 2 O served as solvent treatment control. After treatment for 2 days, cells were subjected to (A) an NLuc assay (mean ± SD, n = 2; ** p < 0.01) and (B) a viral RNA Northern blot assay. (C) CHO‐K1 cells were transfected with BAC‐CoV2‐Rep for 2 days, followed by blasticidin treatment (10 μg/ml) for 8 days. The surviving cells were pooled and treated with remdesivir (10 μM), GC376 (10 μM), or DMSO control for 2 days. The treated cells were lysed and subjected to NLuc assay (mean ± SD, n = 2; * p < 0.05). BAC, bacterial artificial chromosome; CMV, cytomegalovirus; DMSO, dimethyl sulfoxide; RLU, relative luminescence unit; rRNA, ribosomal RNA

Article Snippet: SARS‐CoV‐2 antiviral compounds remdesivir, GC376, and EIDD‐1931 were purchased from SelleckChem.

Techniques: Transfection, Solvent, Control, Northern Blot

Journal: bioRxiv

Article Title: Lassa virus NP DEDDh 3’-5’ exoribonuclease activity is required for optimal viral RNA replication

doi: 10.1101/2023.04.12.536665

Figure Lengend Snippet: (A). Vero cells were treated with 5’-FU and EIDD-1931 at indicated concentrations. At 48 hr post treatment, cell viability was measured using CellTiter-Glo Viability Assay (Promega). Data shown are the average (n=4) and SEM. (B) and (C). Vero cells were treated with 5-FU and EIDD-1931 at different concentrations as indicated. Cells were infected with wt rLASV (wt) and ExoN-rLASV (ExoN-) at MOI 0.1. At 48 hpi, virus titers were determined by plaque assay. Log10 virus titer changes relative to the virus titer of non-treated cells are shown. Data presented are the mean and the SEM of three independent experiments.

Article Snippet: Treatment of Vero cells with EIDD-1931 (5 µM -100 µM) alone did not substantially affect cell viability ( , CellTiter-Glo Viability Assay, Promega).

Techniques: Viability Assay, Infection, Virus, Plaque Assay